SAMHSA Certified Lab

Reference Manual

Rat Serum Protein IgE
ACM Test Number: 10860
Dept: Reference
SPECIMEN COLLECTION REQUIREMENTS
Collection Container:
SST
Transport Temperature: Refrigerated
Specimen Volume: Preferred Volume: 0.5 mL serum; Minimum Volume: 0.3 mL serum
Also Acceptable: Red top Serum
TECHNICAL SPECIFICATIONS
Performed: Monday - Saturday
Reported: 2 Days
Methodology: Fluorescence Enzyme Immunoassay (FEIA)
CPT: 86003
LOINC: 6225-7
Stability: Room Temperature: Unacceptable; Refrigerated: 14 Days; Frozen: 90 Days
Reference Range:
Class       IgE kU/L      Interpretation
0              <0.35          Negative
1              0.35-0.69    Equivocoal
2              0.70-3.49    Positive
3              3.50-17.4    Positive
4              17.5-49.9    Strongly Positive
5              50.0-99.9    Strongly Positive
6              > or = 100   Strongly Positive
Reference Values apply to all ages
Notes: Specific IgE from the patient`s serum reacts with the allergen of interest, which is covalently coupled to an ImmunoCAP. After washing away nonspecific IgE, enzyme-labeled anti-IgE antibody is added to form a complex. After incubation, unbound anti-IgE is washed away and the bound complex is then incubated with a developing agent. After stopping the reaction, the fluorescence of the eluate is measured. Fluorescence is proportional to the amount of specific IgE present in the patient`s sample